Gymnadenia conopsea (L.) R. Br.: Monocotyledoneae, orchid Gymnadenia territorial herb.
Chemical composition
Gymnadenia tuber ethanol extract of n-butanol fraction obtained seven compounds, respectively for hydroxyl benzyl disulfide ether (I) and 4,4 '- hydroxy benzyl sulfoxide (II), adenosine (III), miIharine (IV), loroglossin (V), and dactylorhiu B (VI), dactvlorhin a (VII). Conclusion compounds were isolated from natural products for the first time.
Pharmacological activity
Chinese scholars have studied the pharmacodynamics of the Chinese scholars. It is believed that it can inhibit the surface antigen of hepatitis B virus and promote the proliferation of progenitor cells.
Anti allergic effect
Rirr tuber methanol extract in the passive skin allergy test rat ear (PCA) showed anti allergic activity. The methanol extracts were obtained by reversed phase silica gel column chromatography, and the anti allergic activity was stronger than that of methanol extract, and the efficacy was close to that of anti allergic drug T1-anilast. In rats addicted to alkaline leukemia cells (RBL a 2u3) antigen induced desorption from particles of the inhibitory activity was detected in the methanol elution flow copies of RBL 2H3 antigen induced B caused by amine hexyl glycosidase release inhibitory effect than the methanol extract of strong. In 9 of 10 double bond; GymconopinB in this methanol elution flow of separation, fee and dihydrogen stilbene compounds, and of these two classes of compounds of the RBL 2H3 antigen induced degranulation of inhibitory activity of Yu Chau. The results showed that the activity of phenanthrene and dihydrogen stilbene should have the following requirements: 4 - hydroxy benzyl 3 activity has an important role in the Philippines and dihydro phenanthrene with 4 methoxy radical and 2. 7 12 hydroxy of activity has an important role; or two hydrogen Philippines dimerization of activity had no effect; and 4 hydroxy benzyl dihydrogen stilbene 2 ~ and or 6 enhanced activity.
Antioxidative effect
Compounds involved in the methyl phenol and methyl eugenol has good free radical scavenging activity and on DPPH free of violence sc50 (median elimination concentration) respectively 29.2, 33.4, 8.2, 12.7, 31.2, 15.7, 5.8mol - L. In the superoxide anion radical scavenging assay, these compounds showed significant in vitro antioxidant function.
Inhibition of hepatitis B virus surface antigen
In the inhibition of HBsAg (body infection of hepatitis B virus markers, but also the first in the body of the first detection of viral markers) activity detection, hand
Ginseng extract of four different dose (0.01, 0.03, 0.06, 0.12 mg / L) and eight hemagglutination units of HBsAg (the hepatitis B patients serum) 4h after contact, 0.01 mg / L did not appear to inhibit; 0.03 mg / L 2 times more inhibitory; 0.06, 0.12mg / L is now four times more inhibitory; 0.06, 0.12 mg / L in military participation provided extract and 8 ~ 800 hemagglutination units of HBsAg contact after 4H is still 4 times inhibition; 0.06, 0.12mg / L in ginseng extract and eight hemagglutination units of HBsAg contact 1 ~ 6 h, 2h appeared 4 times more inhibitory. These results showed that the effect of HBsAg was moderate.
Promote the proliferation of progenitor cells
In 60Co - C irradiated mouse erythroid progenitor cells (CFU - E) caused by radiation anemia model in observed group cFU - e training system, the cell number of foci, found in ginseng group and control group, has the role of promoting the proliferation of progenitor cells.